自從2012年CRISPR-Cas9這項(xiàng)基因編輯技術(shù)發(fā)明之后，這項(xiàng)技術(shù)得到了迅速的發(fā)展。它從細(xì)菌防域系統(tǒng)中發(fā)展而來(lái)，可以允許基因材料方便并精準(zhǔn)地被編輯。

然而這項(xiàng)技術(shù)有主要三個(gè)缺點(diǎn)：第一，它代替基因的能力對(duì)于那些復(fù)制的細(xì)胞能夠最有效地發(fā)揮作用。第二，為了讓新材料加入其中它必須首先打破DNA對(duì)，從而產(chǎn)生很多意想不到的作用。第三，這種技術(shù)并不能有效修正點(diǎn)突變。

針對(duì)第三種缺點(diǎn)，其實(shí)也有解決的方法，就是改變某些特別的堿基并且不用切開(kāi)DNA。

Since CRISPR-Cas9, a gene-editing technique has been discovered from 2012, this technique has gone from strength to strength. It was developed from a bacteria defending system and permits genetic material to be edited easily and precisely.

However, it has three flaws. First, its ability of replacing genes works best in those replicating cells. Second, in order to insert new materials into the gap, it has to break the DNA strands first, which will cause undesirable effect. Third, it doesn't work effectly on correcting point mutations. With regard to the third one, there's another way to solve, which can alter specific bases without cutting the DNA strands they are in.