結(jié)果一

主要分群：
namely cancer cells (EPCAM; clusters 2, 5, 10, and 11), fifibroblasts (COL1A1; cluster 7), endothelial cells (CLDN5; cluster 9), T cells (CD3D; clusters 0 and 3), B cells (CD79A; cluster 12), NK cells (NKG7; cluster 4), mast cells (MS4A2; cluster 8), and myeloid cells (LYZ; clusters 1 and 6).

癌細(xì)胞鑒定：
We considered the cells marked by EPCAM were cancer cells referred to a scRNA-seq study of lung cancer（ref：8）

• cancer cells (EPCAM; clusters 2, 5, 10, and 11)
• fifibroblasts (COL1A1; cluster 7)
• endothelial cells (CLDN5; cluster 9)
• T cells (CD3D; clusters 0 and 3)
• B cells (CD79A; cluster 12)
• NK cells (NKG7; cluster 4)
• mast cells (MS4A2; cluster 8)
• myeloid cells (LYZ; clusters 1 and 6)

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結(jié)果二

We identifified 50 different stromal cell subclusters (including 7 clusters from endothelial cells, 5 clusters from fifibroblasts, 17 clusters from T cells, 3 cluster from B cells, 4 cluster from NK cells, 11 clusters from macrophages, and 3 clusters from mast cells) and 13 cancer cell subclusters, of which, we focused on some important cell subclusters to explore their functions.

cancer cell subclusters

stromal cell subclusters：

• 7 clusters from endothelial cells
• 5 clusters from fifibroblasts
• 17 clusters from T cells
• 3 cluster from B cells
• 4 cluster from NK cells
• 11 clusters from macrophages
• 3 clusters from mast cells

結(jié)果三

Compared to normal cells at the bottom of each of the SADC and GGN-ADC groups in Fig. 2a (including endothelial cells, fifibroblasts, T cells, B cells, NK cells, myeloid cells, and mast cells), according to cell chromosome fragment variation, the cells marked by EPCAM were clustered into nine groups.

normal cells：

• endothelial cells
• fifibroblasts
• T cells
• B cells
• NK cells
• myeloid cells
• mast cells

后面開(kāi)始進(jìn)行亞型細(xì)分

1.癌細(xì)胞-13個(gè)亞類以及各自標(biāo)志物

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2.內(nèi)皮細(xì)胞-7個(gè)亞類以及各自標(biāo)志物

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3.成纖維細(xì)胞

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4.T細(xì)胞

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5.B細(xì)胞

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6.NK細(xì)胞

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7.髓系細(xì)胞

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8.肥大細(xì)胞

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文獻(xiàn)來(lái)源：Lu et al. Cell Discovery (2020) 6:69